ANTIBIOTIC ACTIVITY OF WHOLE LEAF EXTRACT OF PHYLLANTHUS EMBLICA ANDHELIANTHUS ANNUUSA GAINST PENICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI.

Abstract

Background:

More than 1 million sexually transmitted infections (STIs) are acquired every day worldwide. The study aims to assess the antibiotic activity of whole leaf extract of Phyllanthus embolic and Helianthus annuus against penicillin-resistant staphylococcus aureus and escherichia coli.

Methodology:

A laboratory-based experimental study was conducted for three months between November 2021 and January 2022. S. aureus and E. coli bacterial isolates from the bio-repository of the microbiology laboratory at the University of Kisubi (UniK) and the diagnostic laboratory at Our Lady of Consolata Kisubi hospital were retrieved and the selected plants were collected from the field; their fresh aqueous leaf extracts were prepared from which the respective antimicrobial susceptibility discs were made

 Results:

S aureus species were resistant to both Phyllanthus Emblica and Helianthus Annuusextracts. There was a higher average resistance to the Helianthus Annuus extract of 94.5% as compared to 90.0% of Phyllanthus Emblica extract. Both susceptibility and resistance profiles were considered depending on the antibiotic cefoxitin (Resistance ˂22≥ Susceptibility) measured in millimeters for zones of inhibition. E. coli species were resistant to both Phyllanthus Emblica and Helianthus Annuus extracts. There was a higher average resistance to the Helianthus Annuus extract of 89.3% as compared to 83.6% of Phyllanthus Emblica extract.

However, both susceptibility and resistance profiles were considered depending on the antibiotic Ampicillin (Resistance ˂14≥ Susceptibility) measured in millimeters for the zones of inhibition

Conclusion:

It was difficult to establish a bioactive-component profile and essential oils contributing towards antimicrobial activity from a specific plant species.

Recommendation:

Detailed qualitative and quantitative studies to distinguish antimicrobial agents in respective whole-leaf extracts need to be conducted and the establishment of therapeutic dosages